Does Bordetella ferment lactose?

Bordetella avium These are non-lactose fermenting, small rods that are characterized by the ability to grow in aerobic conditions.

How do you identify Bordetella bronchiseptica?

They have been reported infected with Bordetella bronchiseptica signs and symptoms including coughing, sneezing, runny nose, fever, anorexia, atrophic rhinitis, acute tracheobronchitis, conjunctivitis, mandibular lymphadenopathy, dyspnea, cyanosis and pneumonia animals.

What media will Bordetella pertussis grow on?

Diagnosis. B pertussis can be cultured on modified Bordet-Gengou medium, charcoal-horseblood agar (Regan-Lowe) or grown in supplement Stainer-Scholte broth.

Is Bordetella bronchiseptica gram positive?

Bordetella bronchiseptica is a well-known Gram-negative bacterial pathogen causing a plethora of diseases in different animals.

How does Bordetella Bronchiseptica grow?

Bordetella can be spread by airborne droplets and is highly contagious. The bacterium does not invade the cells of the respiratory tract nor do they spread to deeper tissues, instead Bordetella colonizes and multiplies on the mucus membranes of the respiratory tract.

What conditions does Bordetella need to grow?

Bordetella organisms grow optimally at 35° to 37° C. Bordetella species are fastidious because their growth can be inhibited by components commonly found in laboratory media. In addition, their rate of growth is inversely related to their degree of fastidiousness.

How does Bordetella bronchiseptica grow?

Is Bordetella catalase positive?

Bordetella are small Gram-negative coccobacilli which are obligate aerobes. They are characteristically catalase and oxidase positive and do not attack carbohydrates.

What specimen is used for culturing Bordetella pertussis?

Identifying which strains of B. pertussis are causing disease is of public health importance. It is best for you to obtain a culture from nasopharyngeal (NP) specimens collected during the first 2 weeks of cough. This is when viable bacteria are still present in the nasopharynx.

Does Bordetella pertussis grow on MacConkey Agar?

B. pertussis does not grow on nutrient agar or MacConkey agar and grows poorly on blood agar. B. pertussis is weakly oxidase positive and is non-motile.

How is Bordetella pertussis identified?

Common laboratory diagnostic methods used for pertussis diagnosis include culture, direct-fluorescent-antibody testing (DFA), serology and polymerase chain reaction (PCR). Culture of Bordetella pertussis is highly specific but fastidious and has limited sensitivity.

What Agar is used to isolate Bordetella pertussis?

Bordet–Gengou agar
Bordet–Gengou agar is a type of agar plate optimized to isolate Bordetella, containing blood, potato extract, and glycerol, with an antibiotic such as cephalexin or penicillin and sometimes nicotinamide.

What does Bordetella pertussis need to grow?

pertussis is the most fastidious and slowest growing of the Bordetella species. Its growth is inhibited by fatty acids, metal ions, sulfides, and peroxides. Isolation of B. pertussis requires a medium containing charcoal, blood, or starch.

Is Bordetella bronchiseptica Gram positive or negative?

Bordetella bronchisepticais a Gram-negative cocco-bacillary aerobe well known for its role in animal disease, particularly kennel cough in dogs (3) and atrophic rhinitis in swine (4).

How do you identify Bordetella colonies on agar?

Colonies isolated on Bordetella selective agar are identified preliminarily by colonial appearance, Gram stain and slide agglutination with polyvalent antiserum. Biochemical and other additional tests are used to distinguish between species of the genus Bordetella and to differentiate Bordetella from similar organisms.

What is Bordetella coccobacilli?

Bordetella are tiny, gram-negative coccobacilli that grow aerobically on starch-blood agar or completely synthetic media with nicotinamide supplemented for growth, amino acids for energy, and charcoal or cyclodextrin resin to absorb fatty acids and other inhibitory substances. Bordetella do not ferment carbohydrates.

Does Bordetella bronchoseptica cause whooping cough?

Bordetella bronchoseptica is a respiratory pathogen of pigs, horses, sheep and dogs and may cause respiratory infections and wound infections in humans. It does not cause whooping cough syndrome. Fears about the toxicity of the whole-cell killed pertussis vaccine have led to an attempt to develop an acellular vaccine.