What is a triple TOF?

The TripleTOF 5600+ system is a hybrid mass spectrometer that combines high performance quadrupole (Qq) mass spectrometry with high resolution Time-of-Flight (TOF) data acquisition and therefore provides a comprehensive platform for discovery and targeted proteomics.

What is a QToF?

QToF is a high-resolution mass spectrometer with the ability to measure accurate mass to charge ratios (m/z) with four decimal places providing unparalleled substance identification.

What is the difference between Qtrap and triple quad?

QTRAP technology delivers equivalent or better data, and more of it, than you can capture on an ordinary triple quadrulpole system. You can combine all the MRM sensitivity of a triple quad, with a multi-functional linear ion trap (LIT), for unique and powerful workflows.

What is the difference between MS and tandem MS?

15.1. A tandem mass spectrometry (TANDEM MS), also named as MS/MS, is a two-step technique used to analyze a sample either by using two or more mass spectrometers connected to each other or a single mass spectrometer by several analyzers arranged one after another.

What is the difference between TOF and QTOF?

Compounds are identified by a combination of their mass, the measured isotope pattern, and chromatographic retention time. Addition of a quadrupole and a collision cell to a TOF forms a QTOF, a more expensive instrument that is able to isolate and fragment specific ions, as well as measure accurate mass.

How much is a Qtof?

Discover and compare Quadrupole Time of Flight Mass Spectrometerd:

What does Qtrap stand for?

The development and introduction of a new generation of LC–MS/MS systems in the form of a hybrid triple quadrupole linear ion trap spectrometers (QTRAP® System) by AB SCIEX in 2002, allows thanks to this technology to combine MRM scan mode with the ion trap scanning functions.

How does a triple quad work?

The triple quadrupole mass spectrometer (TQMS, or QqQ), is a tandem mass spectrometer made up of two quadrupole mass analyzers, with a (non-mass-resolving) radio frequency–only quadrupole between them, acting as a collision cell for collision-induced dissociation (CID) to fragment the selected precursors/parent ions.

What is the major advantage of TOF?

Advantages of using TOF for broad spectrum analysis includes increased mass accuracy and mass resolution, greater sensitivity, rapid acquisition, and increased dynamic range when profiling over a broad molecular weight range.

How much does a triple quad cost?

A triple quadrupole mass spec costs about $350,000, depending on the type of features it comes with, Richardson estimates. A high-resolution time-of-flight instrument can run $350,000 to $400,000.

Is Qtof high-resolution?

High-Resolution QTOF-MRM for Highly Accurate Identification and Quantification of Trace Levels of Triterpenoids in Ganoderma lucidum Mycelium.

How does a Qtrap work?

The system combines the selective scans of the triple quadrupole with the high speed, high sensitivity of the ion trap allowing metabolites to be found and characterized in a single scan. Additionally, the system has MS(3) and time delayed fragmentation scans that aid in structure elucidation.

How does a Paul trap work?

How does a Paul trap work? In simple terms – a Paul trap works by trapping charged particles using electrodynamic fields. This produces a quadrupole potential, described above, which stops the particles escaping, as they are constantly directed towards the bottom of the potential, situated at the centre of the trap.

What is the difference between single quad and triple quad?

Quadrupoles are therefore called mass filters. Single quadrupole systems contain only one mass filtering quadrupole while triple quadrupole systems consist of three quadrupoles.

What does Maldi Tof measure?

During MALDI-TOF analysis, the m/z ratio of an ion is measured by determining the time required for it to travel the length of the flight tube. A few TOF analyzers incorporate an ion mirror at the rear end of the flight tube, which serves to reflect back ions through the flight tube to a detector.

What is TOF analysis?

Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) is a surface-sensitive analytical method that uses a pulsed ion beam (Cs or microfocused Ga) to remove molecules from the very outermost surface of the sample. The particles are removed from atomic monolayers on the surface (secondary ions).

How does a TOF work?

A ToF camera uses infrared light (lasers invisible to human eyes) to determine depth information – a bit like how a bat senses it surroundings. The sensor emits a light signal, which hits the subject and returns to the sensor. The time it takes to bounce back is then measured and provides depth-mapping capabilities.

How much does an HPLC MS cost?

The cost per sample is also based upon the number of samples to be analyzed. As a rough approximation metals analyses usually run between $25 and $75 per sample, and LC/MS/MS and GC/MS/MS analyses are typically between $100 and $200 per sample.

What is the difference between orbitrap and Qtof?

With a QTOF you are already in a range of mass accuracy of about 1-2 ppm and a resolution R = 30000 to 50000. Orbitraps have a mass accuracy in the sub-ppm range and are even better in resolution.

What is trap frequency?

A quadrupole ion trap is a type of ion trap that uses dynamic electric fields to trap charged particles. They are also called radio frequency (RF) traps or Paul traps in honor of Wolfgang Paul, who invented the device and shared the Nobel Prize in Physics in 1989 for this work.

How does a quadrupole work?

The quadrupole consists of four parallel metal rods. Each opposing rod pair is connected together electrically, and a radio frequency (RF) voltage with a DC offset voltage is applied between one pair of rods and the other. Ions travel down the quadrupole between the rods.

What is the difference between ion trap and quadrupole?

A quadrupole filter only measures the ion current for a specific mass-to-charge ratio, while all other mass-to-charge ratios are discarded. In general, an ion trap is more sensitive and can scan a mass range faster than a quadrupole instrument.

How is MALDI-TOF used to identify bacteria?

Identification of microbes by MALDI-TOF MS is done by either comparing the PMF of unknown organism with the PMFs contained in the database, or by matching the masses of biomarkers of unknown organism with the proteome database.

What is the main use of MALDI-TOF in protein studies?

One of the main uses of MALDI-TOF-MS is in the identification of proteins, by peptide mass fingerprinting (PMF). Here we describe a simple protocol that can be performed in a standard biochemistry laboratory, whereby proteins separated by 1D or 2D gel electrophoresis can be identified at femtomole levels.

How does a TOF detector work?

Time-of-Flight (TOF) is a mass analyser that utilises an electric field to accelerate generated ions through the same electrical potential, and then measures the time each ion takes to reach the detector.